Analysis Note

ControlPancreas, Brain

Application

Anti-Somatostatin Antibody, clone YC7 is an antibody against Somatostatin for use in IH.

Immunohistochemistry at 1:50-1:100 on human brain.

Optimal working dilutions must be determined by the end user.

General description

Somatostatin (also known as growth hormone inhibiting hormone (GHIH) or somatotropin release-inhibiting factor (SRIF) has two active forms, 14 amino acids and 28 amino acids, which are produced by alternate cleavage of the single preproprotein. Somatostatin is expressed widely and acts to inhibit the release of several secondary hormones by binding to high-affinity G-protein coupled somatostatin receptors. Presence of somatostatin has been shown in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid.

Immunogen

Synthetic peptide corresponding to amino acids 1-14 of cyclic somatostatin conjugated to bovine thyroglobulin using carbodiimide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Lyophilized Supernatant, preservative free.

Specificity

Recognizes Somatostatin. Shows no cross-reactivity to enkephalins, other endorphins, substance P or CGRP. Partially cross-reacts with somatostatin fragments.

Storage and Stability

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100µL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.